Stewart, who put on 157 in 26 overs with the new one-day captain Adam Hollioake to revive England's innings, was bowled taking a huge swipe at Franklyn Rose because he thought it would be signalled a no-ball by Halley Moore, the umpire.
Moore ignored Stewart's appeal that only three fielders were inside the 30-yard fielding ring - a minimum of four is required in 50-over cricket - and England's coach David Lloyd later had a word with the officials to try to clarify the situation.
Stewart, who had repeatedly gestured to the umpires after being given out, walked off shaking his head in disgust. England's management later said they had accepted the decision. Stewart had hit 14 fours in a 128-ball innings, while Hollioake had immediately re-imposed himself as captain with a 78-ball 76 that included a six out of the ground off Merv Dillon, which took him to his fifty.
The Surrey pair ensured a sizeable total, after a slide to 59 for three. In reply, Gordon Greenidge, 46, and Desmond Haynes, 42, turned back the clock with an opening stand worth 108 before the Vice Chancellor's XI subsided to 207 all out, with West Indies captain Brian Lara run out for nought by Graeme Hick.